Immunoreactive polypeptide profiles of Sarcocystis fusiformis using hyperimmune and naturally infected serum

อังกฤษ

Buffaloes;Bubalus bubalis;Sarcocystis fusiformis;Polypeptides;SDS-PAGE;Western blot

Immunoreactive polypeptide profiles study was made in the whole cyst lysate antigen of Sarcocystis fusiformis which was collected from buffaloes slaughtered at local slaughterhouse, Mathura, U.P. When whole cyst lysate antigen was subjected to SDS-PAGE, twelve major polypeptides (78, 66, 53, 50, 42, 39, 32, 29, 27, 24, 19 and 15kDa) were observed. On western blotting, out of twelve, six (32, 39, 42, 53, 66, 78kDa) and two polypeptides (53kDa and 66kDa) were found to be immunoreactive when probed with homologous hyperimmune and pooled known positive natural serum. No immunoreactivity was seen with pooled known negative natural serum. These two polypeptides (53kDa and 66kDa) bands can be explored for the development of sensitive and specific diagnostic assays.

[1] Amit Singh (Acharya Narendra Deva University of Agriculture and Technology, Uttar Pradesh (India). College of Veterinary Science and Animal Husbandry. Department of Veterinary Parasitology)
[2] Daya Shanker (U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh (India). College of Veterinary Science and Animal Husbandry. Department of Veterinary Parasitology)
[3] Amit Kumar Jaiswal (U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh (India). College of Veterinary Science and Animal Husbandry. Department of Veterinary Parasitology)
[4] Sanjeev Kumar (U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Uttar Pradesh (India). College of Veterinary Science and Animal Husbandry. Department of Veterinary Parasitology)