• อังกฤษ

  • Buffaloes;Bubalus bubalis;Haemorrhagic septicemia;Pasteurella multocida;kmt1 gene;LAMP

  • Pasteurella multocida is an important pathogen affecting livestock and poultry causing significant economic losses. A loop-mediated isothermal amplification (LAMP) assay using four primers targeting a conserved region of the kmt1 gene was standardised to diagnose Pasteurella multocida. The test was carried out at 64 deg C for 45 minutes, the LAMP products could be visually confirmed using fluorescent dye SYBR Green I as detection reagent both with naked eye as well as under UV-illumination. The sensitivity of the developed LAMP assay was 10**(4) fold higher than PCR. Furthermore, no cross-reactivity was found with the other tested bacteria. The developed LAMP assay allows easy, rapid, accurate and sensitive detection of Pasteurella multocida.

  • [1] Sivaramakrishna Akondi (Guru Angad Dev Veterinary and Animal Sciences University, Punjab (India). Department of Veterinary Microbiology)
    [2] Anil Kumar Arora (Guru Angad Dev Veterinary and Animal Sciences University, Punjab (India). Department of Veterinary Microbiology)
    [3] Narinder Singh Sharma (Guru Angad Dev Veterinary and Animal Sciences University, Punjab (India). Department of Veterinary Microbiology)

12

  Export

  ค้นเพิ่มเติม

Sivaramakrishna Akondi. (2022). Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida.  Buffalo Bulletin, 41 (2) ,318-327


Sivaramakrishna Akondi. "Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida" Buffalo Bulletin, 41, 2022, 318-327.

Sivaramakrishna Akondi. (2022). Development and application of a loop-mediated isothermal amplification method for rapid detection of Pasteurella multocida.  Buffalo Bulletin, 41 (2) ,318-327