Polymorphisms in osteopontin gene in Amazon buffaloes

อังกฤษ

Bubalus bubalis;Buffaloes;Amazon buffaloes;Osteopontin gene;Polymorphisms;Identification;SNP;Reproductive traits;Male;Fertility parameters

Aim of this work was to identify polymorphisms in the osteopontin gene. It was used in this experiment 306 male buffaloes, older than 18 months, bred in two farms, one in the State of Amapa and the other farm in the State of Para. There was identified three SNP polymorphisms for the region amplified by the primer OS4 (5 upstream) and four SNP polymorphisms for the region amplified by the primer OS9 (exon 5 to exon 6). The polymorphisms were in positions 1478, 1513 and 1611 in the region amplified by OS4 and positions 6690, 6737, 6925 and 6952 in the region amplified by OS9. These data indicate that the osteopontin gene is important because it can have a substantial influence on the reproductive traits of male buffaloes.

[1] Filho, Sebastiao Tavares Rolim (Universidade Federal Rural da Amazonia, Belem (Brazil))
[2] Ribeiro, Haroldo Francisco Lobato (Universidade Federal Rural da Amazonia, Belem (Brazil))
[3] Camargo, Gregorio Miguel Ferreira De (Universidade Estadual Paulista, Jaboticabal (Brazil))
[4] Cardoso, Diercles Francisco (Universidade Estadual Paulista, Jaboticabal (Brazil))
[5] Aspilcueta-Borquis, Raul Rusbel (Universidade Estadual Paulista, Jaboticabal(Brazil))
[6] Tonhati, Humberto (Universidade Estadual Paulista, Jaboticabal (Brazil))
[7] Nunes, Kim de Borborema (Universidade Federal do Para, Belem (Brazil). Curso de Pos Graduacao em Ciencia Animal)
[8] Vale, William Gomes (Universidade Federal Oeste do Para (Brazil))
[9] Sousa, Keitiane Colares de (Universidade Federal Rural da Amazonia, Belem (Brazil))
[10] Mesquita, Ellen Yasmin Eguchi (Universidade Federal do Para, Belem (Brazil). Curso de Pos Graduacao em Ciencia Animal)
[11] Boaretto, Jose Luiz (Universidade Federal Rural da Amazonia, Belem (Brazil))
[12] Alcantara, Giselia de Lourdes Cardoso de (Universidade Federal Rural da Amazonia, Belem (Brazil))