• อังกฤษ

  • Bubalus bubalis;Buffaloes;Indian Murrah buffalo;Genetic variability;DNA polymorphism;DNA sequences;Production;Milk yield;Milk quality;Fat;Protein;Mastitis;Disease resistance genes;Immune function genes;Body weight gain;Conception rate;Reproduction performance;India

  • Identification of genes of economically important traits and analysis of their polymorphic variants, whose products are responsible for a particular phenotype, may play a key role in assessing the genetic potential for selection of an animal. The polymorphic variants of several genes have been shown to affect the production, reproduction performance and disease resistance in dairy animals. This paper elaborates the detection of genetic polymorphisms in such candidate genes like bovine growth hormone (bGH), kappa casein(CSN3), beta-lactoglobulin(b-lg), Pit-1, leptin (LEP), lactoferrin(Lf), interleukin 8(IL8), toll-like receptor 4 (TLR4) and establish their association with milk production, fat and protein percent, body weight gain, conception rate and incidence of mastitis. Genomic DNA was extracted from whole blood of 150 Murrah buffalo females and amplicons were obtained using specific primers for the genes. Characterization of specific genes was done by custom DNA sequencing. Polymorphism was detected by subjecting the amplicons to PCR-SSCP (LEP and Lf) and PCR-RFLP (bGH, CSN3, b-lg, Pit-1, LEP, IL 8, TLR 4 with a battery of restriction enzymes) analysis. DNA sequences of each gene variant were subjected to Clustal W alignment and variations observed, which revealed a varying degree of variability exhibiting monomorphic as well as polymorphic patterns. Statistical analysis carried out to assess the level of significance for the association of genetic variation with production performance and disease resistance. Since buffalo is less explored specie from molecular genetics point of view, combining information on phenotypic data with molecular data could be the most powerful tool marker assisted selection (MAS) for breed improvement.

  • [1] Archana Verma (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)
    [2] Ishwar Dayal Gupta (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)
    [3] Ravinder Singh Gandhi (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)

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Archana Verma. (2013). Genetic variability in production and immune function genes associated with production traits and incidence of mastitis in Indian Murrah buffalo.  Buffalo Bulletin (Thailand), 32 (Special Issue 2) ,729


Archana Verma. "Genetic variability in production and immune function genes associated with production traits and incidence of mastitis in Indian Murrah buffalo" Buffalo Bulletin (Thailand), 32, 2013, 729.

Archana Verma. (2013). Genetic variability in production and immune function genes associated with production traits and incidence of mastitis in Indian Murrah buffalo.  Buffalo Bulletin (Thailand), 32 (Special Issue 2) ,729