• อังกฤษ

  • Bubalus bubalis;Buffaloes;Murrah buffalo;Genetic polymorphism;CARD 15 gene;Caspase activating recruitment domain 15;Immunity;PCR-RFLP;Genomic DNA;Mastitis;Disease resistance

  • Caspase Activating Recruitment Domain 15 (CARD15) gene is involved in innate immunity and has been identified as one of the candidates for disease resistance. The gene has been mapped in Bovine (BTA18), Human (HSA16) and Mouse (MMU8). Bovine CARD15 gene spans about 30 kb and is comprised of 12 exons, 11 of which are coding. Present study was undertaken with the objectives to identify polymorphism in CARD15 gene coding region and to analyze association between genetic variants with incidence of mastitis in Murrah buffalo. Genomic DNA isolated from whole blood of 150 lactating Murrah buffaloes was amplified using fifteen sets of the primers to cover complete coding region of the gene and the amplicons were subjected to RFLP analysis using BsaH1, PvuII and SacI restriction enzymes (REs). BsaHI-RFLP revealed polymorphism in the 824bp fragment of primer 12.3 exhibiting AA, AB and BB genotypes. SacI RFLP exhibited AB and BB genotypes for primers 1 and 12.1, whereas monomorphic BB genotype for primer 4.1. RFLP patterns using PvuII RE exhibited BB genotype for contig 2, AB and BB for contig 4.2, 4.3 and 5-6 and AA, AB and BB genotypes for contig 12.3. The frequencies for A and B alleles varied from 0.04 to 0.64 and 0.36 to 0.96 respectively indicating B allele to be more prevalent in the population studied. Statistical analysis indicate a significant association between incidence of mastitis and genotypes of P12.3-PvuII (P LT 0.06). Buffaloes with AA genotype were with lower incidence of mastitis in comparison to those with AB and BB genotypes.

  • [1] Indrasen Chauhan (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)
    [2] Archana Verma (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)
    [3] Gupta, I.D. (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)
    [4] Sonawane, Gokul S. (National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division)

80 54

  ไฟล์ดิจิทัล

  Export

  ค้นเพิ่มเติม

Indrasen Chauhan and others. (2013). Genetic polymorphism in caspase activating recruitment domain 15 and its association with incidence of mastitis in Murrah buffalo.  Buffalo Bulletin (Thailand), 32 (Special Issue 2) ,723-728


Indrasen Chauhan and others. "Genetic polymorphism in caspase activating recruitment domain 15 and its association with incidence of mastitis in Murrah buffalo" Buffalo Bulletin (Thailand), 32, 2013, 723-728.

Indrasen Chauhan and others. (2013). Genetic polymorphism in caspase activating recruitment domain 15 and its association with incidence of mastitis in Murrah buffalo.  Buffalo Bulletin (Thailand), 32 (Special Issue 2) ,723-728