Early embryo development in buffalo

อังกฤษ

Buffaloes;Embryo;Morphology;Pregnancy;Fetal stages;Conceptus;Brazil

Aim of this study was to describe the developmental changes in the buffalo conceptuses from 10-60 days of pregnancy using the crown rump methodology to diagnose the estimated age of those samples. Macroscopicexaminations were carried out on 96 buffalo conceptuses, embryo/fetus was measured to determine its age. A stereomicroscope and Scanning Electron Microscopywere usedto describe macroscopic external details of embryos. Samples were photographed to be able to identify main organs like heart, liver, gut, pharyngeal arches and lung, during the development. Fetuses were used for description of ossification points using the Alizarine technique. The obtained results revealed that mammal embryos until 5th weeks of gestation have too much similar characteristics and it is difficult to distinguish the species without checking the placenta features. Similarities between bovine and buffalo persist, except for fetal stages in which buffalos seem to have faster development than that of bovine. In conclusion, the overall data indicated the important characteristics that can be evaluated to verify the viability of buffalo embryos and help the evaluations byultrasonographic exams.

[1] Morini, Adriana Caroprezo (Federal University of Western Para, Para (Brazil). Institute of Biodiversity and Forests)
[2] Ambrosio, Carlos Eduardo (Sao Paulo State University, Sao Paulo (Brasil). Faculty of Animal Science and Food Engineering)
[3] Morini Junior, Joao Carlos (Federal University of Mato Grosso do Sul, Mato Grosso do Sul (Brazil). Faculty of Agronomy)
[4] Pereira, Flavia Verechia Thomaz (Sao Paulo State University. Dracena Campus, Sao Paulo (Brasil). Department of Histology and Embryology)
[5] Vale, William Gomes (Federal University of Western Para, Para (Brazil). Institute of Biodiversity and Forests)
[6] Sousa, Isadora Karolina Freitas De (Federal University of Western Para, Para (Brazil). Institute of Biodiversity and Forests)
[7] Neves, Kedson Alessandri Lobo (Federal University of Western Para, Para (Brazil). Institute of Biodiversity and Forests)
[8] Hamad, Antonio Humberto Minervino (Federal University of Western Para, Para (Brazil). Institute of Biodiversity and Forests)
[9] Miglino, Maria Angelica (Sao Paulo State University, Sao Paulo (Brasil). Faculty of Veterinary Medicine and Animal Science. Department of Surgery)