• อังกฤษ

  • Buffaloes;Individual embryo culture;Two phase atmosphere;Serum-free medium;Predicting;Embryo transfer;Metabonomics;SOFaa;In vitro embryo;China

  • Tracking and predicting the developmental potential of individual embryos has practical importance in research and clinical art, allowing the use of biomarkers in vitro to assess embryo quality as well as predict pregnancy success after embryo transfer. The aim of the present study was to establish an individual buffalo embryo culture system in a modified SOFaa medium without serum and feeder cells under a two phase atmosphere (5 percent CO2 in air), in order to facilitate the metabonomics research for assessing individual embryo quality. Cumulus-oocyte complexes (COCs) from ovaries obtained at local abattoir were selected and cultured for in vitro maturation for 22-24 h in TCM199 medium under 5 percent CO2 in humidified air at 39 deg C. The matured COCs were in vitro fertilized in modified TALP medium. Eight to ten hours after fertilization, the presumptive zygotes were grouped with 15 embryos in a 20 micro L-droplet and co-cultured with feeder cells (buffalo cumulus cell monolayer) in TCM-199 medium at the initial 96 hours. Half volume of medium was replaced 48 hours after culture. After 96 hours, only morula-stage embryos were selected for the following experiment. The morula-stage embryos were divided into two groups. The morula-stage embryos for the experimental group were cultured individually for another 72 hours in 20micro L-droplets of modified SOFaa medium which was modified from the recipe of H. R. Tervit at 1999 while a co-culture system with serum and cumulus cell monolayers was used for the control group. The results showed a slightly lower blastocyst development rate in modified SOFaa comparing to the control group (77/348, 22 percent VS. 63/242, 26 percent). In conclusion, individual culture of buffalo embryos in microdrops of serum-free SOFaa medium can support embryo development to the blastocyst stage. This approach may enable the metabonomics study in individual embryo developmental potential and facilitate the non-invasive assessment of embryo quality in vitro.

  • [1] Di Li (Guangxi University, Guangxi (China). Animal Reproduction Institute. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources)
    [2] Juan Lou (Guangxi University, Guangxi (China). Animal Reproduction Institute. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources)
    [3] Wenxin Zhang (Guangxi University, Guangxi (China). Animal Reproduction Institute. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources)
    [4] Xiaogan Yang (Guangxi University, Guangxi (China). Animal Reproduction Institute. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources)
    [5] Yangqing Lu (Guangxi University, Guangxi (China). Animal Reproduction Institute. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources)
    [6] Kehuan Lu (Guangxi University, Guangxi (China). Animal Reproduction Institute. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources)

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Di Li and others. (2013). A two-step culture system sustains individual buffalo embryo development.  Buffalo Bulletin (Thailand), 32 (Special Issue 2) ,401-404


Di Li and others. "A two-step culture system sustains individual buffalo embryo development" Buffalo Bulletin (Thailand), 32, 2013, 401-404.

Di Li and others. (2013). A two-step culture system sustains individual buffalo embryo development.  Buffalo Bulletin (Thailand), 32 (Special Issue 2) ,401-404