• อังกฤษ

  • Buffaloes;Bubalus bubalis;Trypanosoma evansi;Diagnosis;Latex agglutination;Polymerase chain reaction;India

  • Trypanosoma evansi, a blood protozoan parasite, causes a serious disease known as 'surra', which runs a chronic course in buffaloes. It is an arthropod-borne disease, and Tabanus spp. has been implicated as the main vector. The longterm debilitating effects of parasitic infestations in buffaloes assume greater importance in terms of production losses. Study was conducted to screen the sera and blood samples of buffaloes by monoclonal antibody based latex agglutination test (MAb-LAT) and TE-PCR respectively. Forty-six buffalo serum samples collected from different parts of Haryana were screened by MAb-LAT to detect the circulating antigens of T. evansi and their corresponding blood samples by wet blood film (WBF) and TE-PCR using synthetic oligonulceotide primers (21 mer sense and 22 mer antisense) targeted to a repetitive nuclear DNA sequence of T. evansi. Out of 46 blood and sera samples from buffaloes, 2 percent were positive by WBF, 78.26 percent positive by MAb-LAT and 76.09 percent by TE-PCR. Diagnostic sensitivity of MAb-LAT was found to be more than WBF and PCR. MAb-LAT, being simple to perform, rapid, convenient, and cost-effective, could be quite suitable for field-level diagnosis. Thus MAb-LAT provides a better tool for the early diagnosis and thereby effective control of trypanosomosis, which leads to improved productivity in buffaloes.

  • [1] Shyma, K.P. (Indian Veterinary Research Institute, Uttar Pradesh (India). Division of Parasitology)
    [2] Gupta, S.K. (Chaudhari Charan Ssingh Haryana Agricultural University, Haryana (India))
    [3] Ajit Singh (Chaudhari Charan Ssingh Haryana Agricultural University, Haryana (India))
    [4] Chaudhri, S.S. (Chaudhari Charan Ssingh Haryana Agricultural University, Haryana (India))

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Shyma, K.P. and others. (2012). Efficiency of monoclonal antibody based latex agglutination test in detecting Trypanosoma evansi under field conditions for improving the productivity in buffaloes.  Buffalo Bulletin (Thailand), 31 (3) ,163-172


Shyma, K.P. and others. "Efficiency of monoclonal antibody based latex agglutination test in detecting Trypanosoma evansi under field conditions for improving the productivity in buffaloes" Buffalo Bulletin (Thailand), 31, 2012, 163-172.

Shyma, K.P. and others. (2012). Efficiency of monoclonal antibody based latex agglutination test in detecting Trypanosoma evansi under field conditions for improving the productivity in buffaloes.  Buffalo Bulletin (Thailand), 31 (3) ,163-172