Oestrone sulphate microtiterplate enzymeimmunoassay in plasma of swamp buffalo during induced oestrous cycles and in milk of cattle during early pregnancy

อังกฤษ

OESTROUS CYCLE;ENZYME IMMUNOASSAY;PREGNANCY;SWAMP BUFFALO;MILK

Development of a microtiterplate enzymeimmunoassay for direct oestrone sulphate (E1S) determination in both plasma and milk was described. In buffalo, plasma profiles of E1S during oestrous cycle were around days of oestrus and 5 to 14 of the cycle with the increasing levels of 0.2-0.5 ng/ml. In dairy cattle, the mean E1S levels at early pregnancy of a pregnant group was higher than in the non-pregnant, abortive and subfertile groups as the levels at day 20 of 7.1, 3.2, 1.9 and 1.5 ng/ml respectively and at day 28 of 7.9, 4.3, 1.3 and 2.5 ng/ml respectively. Indentification of confidential limit in a number of pregnant animals showed significantly higher levels than the non-pregnant, abortive and subfertile animals was obtained at 50 percent. The result suggested that E1S in maternal circulation may be only one of the other unknown factors to promote conception at a very early stage of pregnancy.

[1] Kamonpatana, M. (Chulalongkorn University Henri Dunant Street, Bangkok 10500 (Thailand). Biochemistry Unit, Faculty of Veterinary Science)
[2] Srisakwattana, K. (Chulalongkorn University Henri Dunant Street, Bangkok 10500 (Thailand). Biochemistry Unit,)
[3] Pansin, C. (Chulalongkorn University Henri Dunant Street, Bangkok 10500 (Thailand). Biochemistry Unit,)